DVM Student North Carolina State University Raleigh, NC, United States
Abstract: Background The effect of storage on hematologic and biochemical properties of caprine blood products is not well-understood. Objectives Assess the impact of storage duration on erythrocyte fragility, packed cell volume (PCV), hemolysis and biochemical composition in caprine whole blood (WB) and packed red blood cells (pRBCs). Animals 6 healthy Boer x Kiko goats Methods Repeated measure cohort study. The WB and pRBC were stored in CDPA-1 (citrate, dextrose, phosphate, adenine) or CPD (citrate, phosphate, dextrose) with Optisol, respectively, at 4-7°C for seven weeks. The PCV, osmotic fragility, glucose, and lactate concentrations were measured weekly, and hemolysis and potassium concentrations were measured bi-weekly. Changes from baseline were assessed with ANOVA or Friedman's test and Dunnett’s or Dunne’s correction for multiple comparisons. P-values < 0.05 were considered significant. Results Median PCV decreased from 31% to 21.5% after two weeks in WB (P=0.002), with no change in pRBC. Significant hemolysis was observed after two weeks in WB (P=0.04) and pRBC (P=0.02), while osmotic fragility did not increase in either product. Baseline glucose (~500 mg/ml) decreased by two and three weeks in pRBC (P=0.002) and WB (P=0.0005), respectively, but remained substantially higher (>300 mg/dL) than in normal blood. Lactate concentrations increased after one and three weeks in WB (P=0.045) and pRBC (P=0.03), respectively. Potassium concentrations increased substantially after two weeks (P=0.001) in both products. Conclusions and Clinical Importance Hemolysis and biochemical changes in caprine blood products develop as early as one week. The use of fresh blood may be preferable for critically-ill patients.
